The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Glucosamine,
D-Glucosamine sulphate and N-Acetyl Glucosamine in food
supplements, foodstuffs, beverages and other materials

Principle:
(desulphation)
(1) Glucosamine sulphate → D-glucosamine + sulphate

(deacetylation)
(2) N-Acetyl Glucosamine → D-glucosamine + acetate

(hexokinase)
(3) D-Glucosamine + ATP → D-glucosamine-6-P + ATP

(glucosamine 6-phosphate deaminase)
(4) D-Glucosamine-6-P + H2O → D-fructose-6-P + NH4+

(phosphoglucose isomerase)
(5) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(6) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

	Kit size:  50 assays (manual) / 500 (microplate)
                                          / 500 (auto-analyser) Method:  Spectrophotometric at 340 nm Reaction time:  ~ 8 min Detection limit:  1.33 mg/L Application examples: Food supplements, food products and beverages Method recognition:  Novel method 

Advantages

• Novel product with simple format
  
• All reagents stable for > 2 years after preparation
  
• All enzymes supplied as stable suspensions
  
• Very rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats